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Preliminary Screening of Hydro-ethanolic Extract of Pothos scandens L for its Potential Anticancer Activity

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Pothos scandens L is a medicinal aroid which has been used traditionally by people for curing many diseases including epilepsy, convulsions and cancer. The present study was carried out to screen the potential anticancer activity of 50% hydro-ethanolic extract of Pothos scandens L (HEEPS). In the present study evaluation of phytochemical constituents of aerial parts of Pothos scandens L using various extracting solvents viz- petroleum ether, chloroform, acetone, methanol, ethanol and water, its antioxidant activity using Ferric Reducing Antioxidant Power (FRAP) assay and evaluation of its potential cytotoxic activity using LDH leakage analysis and DNA fragmentation assay in MCF-7 breast cancer cell lines were carried out. The results revealed that presence of phyto-constituents were more in ethanolic extract when compared to others. The FRAP value of HEEPS was found to be 187.49 ± 11.32 mmol Fe2+/g extract. The LDH leakage assay showed significant cytotoxic activity against MCF-7 cell lines. Further visible fragmentation of DNA was observed in MCF-7 cell lines treated with HEEPS. From the results obtained, it can be concluded that Pothos scandens can be considered as a potential candidate for anticancer drug research.

Keywords:Anticancer, FRAP assay, LDH leakage assay, MCF-7 breast cancer cell lines, Pothos scandens L.


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